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. 2020 Oct 7;10:16656. doi: 10.1038/s41598-020-73340-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Proangiogenic effects of N-NAM in a reconstituted tissue model of angiogenesis. (a) Matrigel was mixed with 1 µM of N-NAM, scN-NAM, or PBS and injected subcutaneously into the mid-ventral region of BALB/c mice. Plugs were harvested 12 days later and subjected to a hemoglobin assay and immunofluorescent staining. (b) Representative images of Matrigel after harvesting. (c) Hemoglobin content was measured and normalized versus the Matrigel weights in the plugs. Results are presented as the means ± SEMs (n = 3). (d,e) Plugs were embedded in paraffin, and sections were prepared and stained with anti-CD31 antibody. The number of CD31-positive cells was counted and the results are presented as the means ± SEMs (n = 3). **p < 0.01, *p < 0.05 versus PBS-treated controls, ^##p < 0.01 between indicated groups.