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. 2020 Sep 24;11:480. doi: 10.3389/fendo.2020.00480

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Copyright © 2020 Tratwal, Bekri, Boussema, Sarkis, Kunz, Koliqi, Rojas-Sutterlin, Schyrr, Tavakol, Campos, Scheller, Sarro, Bárcena, Bisig, Nardi, de Leval, Burri and Naveiras.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Red-to-yellow transition with aging. Representative H&E images of femurs (A) tibiae (C). Note high adiposity on proximal and distal but not mid-shaft of 18-months-old female tibia. MarrowQuant analysis for (B) femurs (n = 3) and (D) tibiae (n = 3) from 8-weeks-old and 18-months-old C57BL/6 females. BM adiposity is significantly higher in 8-weeks-old tibias vs. femurs (P = 0.0045) and in 18-months-old tibias vs. femurs (P = 0.0316). Hematopoiesis is greater in 18-months-old femurs than tibias (P = 0.0993). Error bars represent mean ± s.d. P-values are indicated for hematopoietic compartment (purple) and adipocytic compartment (yellow) are ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001 by Student's t-test for cellularity (black) and adiposity (gray). (E) BMAd fragmentation distribution of the proximal (purple) and distal (orange) 8-weeks-old tibia (n = 10) vs. (F) the 18-months-old tibia (n = 3). ^***P < 0.001 by multiple comparisons test. Representative mid-longitudinal H&E section of bone marrow compartments in the proximal-to-distal caudal vertebrae of (G) 8-weeks-old and (H) 18-months-old mice. MarrowQuant analysis of caudal vertebrae in (I) 8-weeks-old (n = 6) and (J) 18-months-old (n = 4) female mice. ^****P < 0.0001, ^**P < 0.01 for cellularity and adiposity and ^*P < 0.05 for cellularity by two-way ANOVA. Mice were C57BL/6 females housed at room temperature and fed a standard ad libitum chow diet. BM, bone marrow; BMAd, bone marrow adipocyte; H&E, hematoxylin and eosin; mo, month; wk, week.