TABLE 2.
Summary of the antibodies and lectins used, including species of elaboration, dilution, catalogue number, and manufacturer
| Antibody | 1st Ab species/dilution | 1st Ab catalogue number | 2nd Ab species/dilution, catalogue number |
|---|---|---|---|
| Anti ‐ Gαo | Rabbit 1:200 | Santa Cruz Biotechnology sc‐387 | ImmPRESS VR HRP anti‐rabbit IgG Reagent MP‐6401‐15 |
| Anti ‐ Gαo | Rabbit 1:400 | MBL 551 | ImmPRESS VR HRP anti‐rabbit IgG Reagent MP‐6401‐15 |
| Anti ‐ Gαi2 | Rabbit 1:200 | Santa Cruz Biotechnology sc‐7276 | ImmPRESS VR HRP anti‐rabbit IgG Reagent MP‐6401‐15 |
| Anti ‐ OMP | Goat 1:400 | Wako 544‐10001 | Horse anti‐goat IgG 1:250 Vector BA‐9500 |
| Anti ‐ GAP‐43 | Mouse 1:400‐1:4,000 | Sigma G9264 | ImmPRESS VR HRP anti‐mouse IgG Reagent MP‐6402‐15 |
| Anti ‐ CB | Rabbit 1:6,000 | Swant CB38 | ImmPRESS VR HRP anti‐rabbit IgG Reagent MP‐6401‐15 |
| Anti ‐ CR | Rabbit 1:6,000 | Swant 7697 | ImmPRESS VR HRP anti‐rabbit IgG Reagent MP‐6401‐15 |
| Anti‐α‐tubulin | Rabbit 1:500 | Abcam 7291 | ImmPRESS VR HRP anti‐mouse IgG Reagent MP‐6402‐15 |
| UEA | 60 µg/ml | Vector L‐1060 | Rabbit 1:50 DAKO P289 |
| LEA | 20 µg/ml | Vector B‐1175 | Vectastain ABC reagent PK‐4000 |
| BSI‐B4 | 100 µg/ml | Sigma L‐2140 | Vectastain ABC reagent PK‐4000 |
ABC, avidin‐biotin‐complex; BSI‐B4, Bandeiraea simplicifolia isolectin B4; CB, calbindin; CR, calretinin; GAP‐43, growth‐associated protein 43; Gαi2, subunit αi2 of G protein; Gαo, subunit αo of G protein; HRP, horseradish peroxidase; IgG, Immunoglobulin G; LEA, Lycopersicum esculentum agglutinin; OMP, olfactory marker protein; UEA, Ulex europaeus agglutinin.