TABLE 2.
Plasmids used in this study
| Plasmid | Description | Source or reference |
|---|---|---|
| pET28a(+) | His-tagged cloning vector for protein purification; Kanr | Novagen |
| pJSpe | Modified pJOE8999 optimized for Gibson assembly of homology templates; Ampr Kanr | 25 |
| pPT037 | pJSpe::cheV (for cheV knockout) | 25 |
| pPT058 | pJSpe::mcpB (for mcpB knockout) | 25 |
| pPT053 | pJSpe::hemAT (for hemAT knockout) | This work |
| pAIN750 | B. subtilis empty vector for integration at amyE; Ampr Spcr | 98 |
| pPT200 | pAIN750::mcpB[M1-V287] mcpA[L287-E661] | This work |
| pPT205 | pAIN750::mcpB[M1-Q359] mcpA[D359-E661] | This work |
| pGB42 | pAIN750::mcpB[M1-A374] mcpA[S374-E661] | This work |
| pGB43 | pAIN750::mcpB[M1-N397] mcpA[E397-E661] | This work |
| pGB34 | pAIN750::mcpB[M1-I433] mcpA[Q433-E661] | This work |
| pGB64 | pAIN750::mcpB[M1-Q423] mcpA[A423-E661] | This work |
| pGB35 | pAIN750::mcpB[M1-L481] mcpA[R481-E661] | This work |
| pPT086 | pAIN750::mcpA[M1-Q358]-mcpB[D359-E662] | This work |
| pGB65 | pAIN750::mcpB[A431S] | This work |
| pGB83 | pAIN750::mcpB[T424A] | This work |
| pGB82 | pAIN750::mcpB[D427T] | This work |
| pGB67 | pAIN750::mcpB[E581Q] | This work |
| pGB79 | pAIN750::mcpB[K585E] | This work |
| pGB94 | pAIN750::mcpB[E399K] | This work |
| pPT262 | His6-C-terminal McpB expression plasmid, pET28(a)::mcpBC | This work |
| pGB78 | His6-C-terminal McpBC-A431S expression plasmid, pET28(a)::mcpBC[A431S] | This work |
| pGB53 | His6-C-terminal McpA expression plasmid, pET28(a)::mcpAC | This work |
| pGEX-6p-2::cheA | GST-CheA overexpression plasmid | 22 |
| pGEX-6p-2::cheW | GST-CheW overexpression plasmid | 22 |
| pGEX-6p-2::cheD | GST-CheD overexpression plasmid | 22 |
| pGB46 | His6-C-terminal HemAT expression plasmid, pET28(a)::hemATS | This work |
| pSP03 | His6-N-terminal HemAT expression plasmid, pET28(a)::hemATN | This work |