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. 2020 Oct 6;11(5):e02315-20. doi: 10.1128/mBio.02315-20

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Copyright © 2020 Jiang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — A D102R substitution in Z2B3 can restore the reactivity to Serbia N1. (A and D) Gel filtration survival analysis of Z2B3-D102R Fab with Serbia N1 (A) and 18N1 (D). Z2B3-D102R (magenta), NA (cyan), or Z2B3-D102R-NA (blue) mixtures were analyzed by a Superdex 200 gel filtration column. The elution fractions were pooled and analyzed by SDS-PAGE. (B and E) An SPR assay characterizing the specific binding between Z2B3-D102R and specific NAs, including Serbia N1 (B) and 18N1 (E). The binding affinity (K_D) values were calculated using a 1:1 Langmuir model produced with BIAcore 3000 analysis software (BIAevaluation version 4.1). (C and F) NA inhibition activity of Z2B3 and Z2B3-D102R for Serbia N1 (C) and 18N1 proteins (F).