Fig. 1.

Quality comparison between EM-seq and WGBS. Boxplots showing: a mapping rates of EM-seq and WGBS. b duplication rates of EM-seq and WGBS. c read filtering rates of EM-seq and WGBS. Reads with three or more consecutive methylated CHH sites are considered as non-converted reads and removed from subsequent analyses. d Boxplot showing average coverages of methylated cytosines in EM-seq and WGBS. In a–d, blue box represents EM-seq and red box represents WGBS. The P values indicated at the top of each plot were estimated using a Student’s t-test