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. 2020 Oct 7;10:16669. doi: 10.1038/s41598-020-73704-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of ADH1 and PDC1 in aerobic and waterlogged Arabidopsis plants. (a) GUS staining of pADH1:GUS plants in air or 5-day waterlogging. (b) Comparison of the expression (RT-qPCR) of ADH1, PDC1, and PDC2 in roots and shoots of aerobic plants. (c) Comparison of the expression (RT-qPCR) of ADH1, PDC1, and PDC2 in air (green box) vs. 5-day waterlogging (WL, blue box). Lines in the boxes indicate the median. The bottom and top of each box denote the first and third quartile, respectively. The dots represent the single data points and whiskers denote the min/max values. Statistically significant differences are indicated by an asterisk (T test, pairwise comparison at each time point, * = p < 0.05; ** = p < 0.01; *** = p < 0.001; **** = p < 0.0001). (d) Immunoblot analysis of proteins in aerobic and waterlogged plants. Equal loading was verified by protein staining (Figure S4).