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. 2020 Oct 7;10:16725. doi: 10.1038/s41598-020-73270-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Radiographic evidence of OA in control (n = 9) and RCCL (n = 30) groups, scored by three independent observers. (A) Global OA score on the scale of 0–3; (B) joint effusion score on the scale of 0–2; (C) osteophytosis score on the scale of 0–3; (D) intra-articular mineralization score on the scale of 0–2; E) medial buttress score on the scale of 0–1 and (F) caudal tibial shelf score on the scale of 0–1. Data are displayed as box-and-whisker plots representing the first and third quartiles, median and range of concentrations for each score. Dotted line: RCCL vs. control threshold obtained by receiver operating characteristic (ROC) curve. ** p < 0.01, *** p < 0.001, **** p < 0.0001 for Wilcoxon test, α = 0.05.