Skip to main content
. 2020 Oct 7;10:16755. doi: 10.1038/s41598-020-73785-8

Figure 3.

Figure 3

miR-522-3p expression is associated with paclitaxel sensitivity in ovarian cancer cell lines. (A) Cells were transfected with pre-miR-522-3p or control-miR, and the expression of miR-522-3p relative to RNU6B was calculated 24 h later using miRNA quantitative reverse transcription polymerase chain reaction (RT-qPCR) and the 2-ΔΔCT method (results shown as relative fold differences). (B) At 24 h after transfection with miR-522-3p or control-miR, the cells were treated using paclitaxel for 48 h (HeyA8-PR) or 72 h (SKOV3ip1-PR) and then the cell viability was assessed based on the MTS assay. (C) Cells were transfected with anti-miR-522 or control-miR for 24 h before miRNA RT-qPCR was performed. (D) At 24 h after transfection with anti-miR-522 or control-miR, the cells were treated using paclitaxel for 48 h (HeyA8) or 72 h (SKOV3ip1), and then cell viability was assessed based on the MTS assay. Experiments were performed in triplicate. Data are presented as mean ± standard deviation from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.