Figure 4.

Effect of EA pretreatment on m-calpain expressed in the rat hippocampal CA1 neurons (n = 4). The expression and cell localization of m-calpain at 24 h after cerebral I/R were detected by WB and double-standard immunofluorescence staining. (a) Representative WB bands showing m-calpain expression in rats among the three groups. (b) Comparison of the m-calpain expression. The result did not conform to the normal distribution. Differences among groups were examined using the Kruskal-Wallis test (H = 21.421, P < 0.001) followed by the Dunn test for post hoc multiple comparisons. (c) Representative double immunofluorescence staining (yellow) of m-calpain-positive cells (red) and NeuN-positive cells (green) in brain sections. Scale bars = 100 μm. ^∗P vs. sham; ^#P vs. MCAO/R.