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Nintedanib attenuates radiation-induced pulmonary cell fibrogenic differentiation. (a, b) LSCs and LSC-differentiated alveolar cells were exposed to 4 Gy followed by treatment with or without nintedanib (1 μM) and then incubated for 3 days. The levels of SP-C (red) and α-SMA (green) protein were determined by coimmunostaining. The arrowheads indicate SP-C+/α-SMA+ cells. The nuclei of cells were stained blue with DAPI. Scale bars, 75 μm.
