FIG 3.

Viral particle recovery from built environment surface materials. (A) Image of the inoculated plates (BSS, PSS, FRP, and PETG) where inactivated viral particles (10 μl of NATtrol) were aliquoted 10 times onto four separate materials in triplicate. (B) Viral particles were either kept overnight at room temperature as liquid (Eppendorf tube was closed; Water No Swab, purple triangles) or desiccated overnight at room temperature in tubes (No Swab Desiccated, red circles) which were then sacrificed to extract RNA directly without removing from the surface. In addition, aliquots of viral particles that were not desiccated but inoculated in DRS and swab materials were also processed (DRS Swab, blue squares). (C) Viral particles were collected from the seeded surfaces with Isohelix swabs and DRS, extracted on the Maxwell RSC, and quantified using RT-qPCR assay. Viral RNA copy number for each condition was divided by an extraction control to calculate percent recovery for day 1 (blue inverted triangles), day 2 (red circles), and day 8 post-bleach (green squares). Statistical significance was determined by Welch’s t test with significance (P < 0.05) denoted by asterisks.