Table 3. Intrinsic Affinity (Apparent K_d, μM) of 14-3-3·Phospholigand Complexes^a,b.

14-3-3 isoform	ERα-ctp (1)	Task3-ctp (2)	GpIBα-ctp (3)
β	0.72 ± 0.1	2.4 ± 0.2	18 ± 3
ε	3.4 ± 0.2	6.0 ± 0.4	28 ± 5
ζ	1.5 ± 0.2	1.3 ± 0.1	20 ± 2
σ	6.6 ± 0.6	2.1 ± 0.1	47 ± 6
τ	1.1 ± 0.1	2.2 ± 0.2	20 ± 3

^aReported K_d (μM) values represent the average of two independent experiments.

^bThe [14-3-3] was varied from 40 nM to 160 μM; [phospholigand] = 100 nM.