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. Author manuscript; available in PMC: 2020 Oct 8.
Published in final edited form as: Structure. 2019 Feb 7;27(4):679–691.e14. doi: 10.1016/j.str.2019.01.005

Figure 5. Analysis of Tomograms.

Figure 5.

(A) The isosurface of the subtomogram average density map of pattern 4 from A. longum, fitted with the known structure of the GroEL (PDB: 1KP8).

(B) Assessment of pattern 1 detected in tomogram of B. bacteriovorus cell. Box plot of the distribution of alignment scores of the subtomograms of pattern 1 against all different template complexes (denoted by PDB code). The complexes are ordered according to median score in descending order. One-sided Wilcoxon rank-sum test with p-value < 1.7 × 10−24.

(C) (Red) box plot of the alignment score distribution of subtomograms in pattern 1 (B. bacteriovorus) against the ribosome template complex (PDB: 2J00–2J01). (Blue) box plot of the alignment score distribution of all other extracted subtomograms against the ribosome template. One-sided Wilcoxon rank-sum test with p-value < 6.3 × 10−6.

(D) A thin section of embedded instances of different patterns, outlined by embedded instances of membrane patterns from tomogram of B. bacteriovorus cell. Left panel: a slice of tomogram. Shown are all membrane patterns in yellow. Second panel from left: patterns 0, 1, 9 containing ribosome structures. Third panel from left): pattern 6. Fourth panel from left: patterns 4 and 5. For analysis of patterns from tomograms of A. longum and H. gracilis, see Figure S3.