Prolonged neuroinflammation after TBI (7 dpi) was abrogated by microglial elimination. As above, mice were provided diets formulated with either vehicle or PLX5622 for 14 days and were sham-injured (control) or were subjected to midline fluid percussion injury (TBI). At 7 dpi (21d of Veh or PLX diet), the cortex was dissected, flash-frozen, and RNA was extracted. mRNA copy number of 262 genes was determined by NanoString nCounter mouse inflammation v2 panel plus (n = 4–8). Genes associated with microglia, chemokines/cytokines, injury, complement, or interferon are represented. Data are presented as average copy number ± SEM. Means with (*) are different from Veh-CON (p-adj < .05) and means with means with (#) are significantly different from Veh-TBI (p-adj < .05)