FIGURE 7.

Microglia–neuronal interactions were heterogeneous in the somatosensory cortex 7 dpi. Thy1-YFP-H mice were uninjured (control) or were subjected to midline fluid percussion injury (TBI). At 7 dpi, brains were perfused, fixed, sectioned, and labeled for P2RY12 (red). Confocal imaging was used to visualize P2RY12 labeling and YFP expression (green). (a) Representative merged images (63×) of P2RY12 (red) and YFP expression (green) from the somatosensory cortex of control mice. Arrows show small P2RY12⁺ microglia (arrows) near YFP⁺ neurons. (b) Representative merged images (63×) of P2RY12 (red) and YFP expression (green) from the somatosensory cortex of TBI mice 7 days after injury. Arrows depict the alignment of P2RY12⁺ rod microglia with YFP⁺ apical dendrites. (c) Representative merged images (63×) of P2RY12 (red) and YFP expression (green) from the somatosensory cortex of TBI mice 7 days after injury. Arrows highlight rod microglia aligned with YFP⁺ axons of pyramidal neurons. (d) Representative merged images (63×) of P2RY12 (red) and YFP expression (green) from the somatosensory cortex of TBI mice 7 days after injury. Arrows denote microglia surrounding the soma of YFP⁺ neurons in cortical layer V