FIGURE 8.

Elimination of microglia with the CSF1R antagonist PLX5622 attenuated rod microglial formation in the somatosensory cortex after TBI. (a) Adult C57BL/6 mice were provided diets formulated with either vehicle (Veh) or PLX5622 (PLX) for 14 days. Next, mice were uninjured (control) or were subjected to midline fluid percussion injury (TBI). At 7 dpi (21d of Veh or PLX diet), brains were perfused, fixed, sectioned, and labeled for 1ba1 (green). (b) Representative images of Iba1 labeling (20×) in the somatosensory cortex 7 dpi. Inset indicates region used for analysis. (c) Total number of Iba1⁺ cells per 20× field in the somatosensory cortex of mice subjected to control or TBI on Veh or PLX diet (n = 4). (d) Number of Iba1⁺ rod microglia per 20× field in the somatosensory cortex of mice subjected to control or TBI on Veh or PLX diet (n = 4). Bars represent the mean ± SEM. Means with (*) are significantly different from controls