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. 2020 Oct 7;8(2):e000973. doi: 10.1136/jitc-2020-000973

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© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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Oral administration of AKK improved gut barrier function and commensal microbiota homeostasis under IL-2 treatment in tumor-bearing mice. (A) Representative images of H&E and AB-PAS staining of the colon tissues and immunofluorescence staining of the mucus in the small intestine. (B) 3D-Principal coordinate analysis (3D- PCoA) of fecal samples (Bray-Curtis distances), followed by Adonis test (*p<0.05). (C) Observed richness (Sobs) index. (D) Shannon diversity index. All data are shown as mean±SD (n=6) (*p<0.05, **p<0.01). AB-PAS, alcian blue-periodic acid Schiff; AKK, Akkermansia muciniphila; DAPI, 4’,6-diamidino-2-phenylindole; IL-2, interleukin 2; OTU, operational taxonomic unit.