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Workflow and implementation of aBASE: After quality control, PCR-amplicon sequencing files are parsed, blasted against germline VDJ-genes using IgBLAST, and blasted against constant gene parts using BLAST. Output is used to generate cloning recommendations and written to a human-readable database. FACS = fluorescence activated cell sorting. CDR = complementarity determining region. SHM = somatic hypermutations. Ig = immunoglobulin
