Table 3.

Specific PCR used in this study

Genes	Primers	Amplification parameters
NHM(PCR1)	NlpC-F-SOE: CAAGGAGGAAAAGACCACATGGTAGATGCAAAGAAAGTATTG	95 °C for 5 min, 95 °C for 30 s, 57 °C for 30 s, and 72 °C for 60 s
	NlpC-R: GGAAGATCTCCATGGCTCGAGATGATGATGATGATGGTGTAGTGAAGGACGAACAGC
PslpA(PCR2)	PslpA-F: CCGGAATTCAAGCGGTAGGTG	95 °C for 5 min, 95 °C for 30 s, 57 °C for 30 s, and 72 °C for 40 s
	PslpA-R-SOE: CAATACTTTCTTTGCATCTACCATGTGGTCTTTTCCTCCTTG
PslpA-NHM(SOE-PCR)	PslpA-F: CCGGAATTCAAGCGGTAGGTG	95 °C for 5 min, 95 °C for 30 s, 56 °C for 30 s, and 72 °C for 100 s
	NlpC-R: GGAAGATCTCCATGGCTCGAGATGATGATGATGATGGTGTAGTGAAGGACGAACAGC
aiiK(PCR3)	aiiKF: GGAAGATCTATGTGTCAAAATAAAAAGTTGTAC	95 °C for 5 min, 95 °C for 30 s, 54 °C for 30 s, and 72 °C for 60 s
	aiiKR: CGGGGTACCTTATTCGTAATACCCTTCCGTTGA
Sequencing	EcoRI-PSlpA-F: CCGGGAATTCAAGCGGTAGGTGAAATATTAC	95 °C for 5 min, 95 °C for 30 s, 55.5 °C for 30 s, and 72 °C for 120 s
	BamHI-T-R: GGCCGGATCCAGCTTGCGTTTGATTTTC

His tag was marked in italics, and multiple cloning sites (MCS) were marked in bold