Fig. 5.

Effect of the miR-361-3p/SERT axis on serotonin-induced upregulation of p-ERK1/2 and the signaling diagram of this study. a The level of t-ERK1/2 and p-ERK1/2 in the hPASMCs of the blank group (no treatment), serotonin group (treated with 250 μmol/l serotonin for 48 h), serotonin + miR-NC group (after transfection with miR-NC for 24 h, treated with 250 μmol/l serotonin for 48 h), and serotonin + miR-361-3p group (after transfection with miR-361-3p mimic for 24 h, treated with 250 μmol/l serotonin for 48 h). b The level of t-ERK1/2 and p-ERK1/2 in the hPASMCs of the miR-NC + pcDNA group (transfected with miR-NC and empty pcDNA3.1 + plasmid), miR-361-3p + pcDNA group (transfected with miR-361-3p mimic and empty pcDNA3.1 + plasmid), and miR-361-3p + ov-SERT group (transfected with miR-361-3p mimic and ov-SERT). c The signaling diagram indicates that miR-361-3p plays its role by suppressing the translation of SERT mRNA to reduce the intracellular accumulation of serotonin, consequently inhibiting the ERK1/2 signal