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. 2020 Oct 7;21:700. doi: 10.1186/s12864-020-07115-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — KASP assays of SNP sex association. Details for the 12 markers tested by KASP assays for the significant GW QTLs of sex determination. From top to bottom: 12 marker IDs; SNP type and SNP position in the genetic map in cM; KASP assays results of 200 remaining individuals and 2 parents and 46 wild individuals. The outer circle diameters for the KASP assay results are proportional to the number of alleles successfully tested; the inner disks represent the genotype of the markers consistent with phenotypic sex based on the ddRAD-Seq; percentage = the inner disk / the outer circle. Detailed data are provided in Table S6. nnxnp: homozygous ♂ and heterozygous ♀; hkxhk: heterozygous ♂ and heterozygous ♀; lmxll: homozygous ♀ and heterozygous ♂