Fig. 4.

Effects of ACE production of inflammatory mediators in stimulated primary peritoneal macrophages. Thioglycollate (3%), rIFN-γ (10 U/mL), and LPS (1 μg/mL)-stimulated primary peritoneal macrophages were incubated for 48 h. a NO and (b) PGE₂ level were measured with Griess reagent and the EIA kit, respectively. NIL (20 μM) or NS398 (5 μM) was used as positive control. Values are presented as mean ± S.D. of three independent experiments. ^###p < 0.001 when compared with control; ^*p < 0.05, ^***p < 0.001 when compared with LPS-induced group. Significant differences between treated groups were determined by ANOVA and Dunnett’s post hoc test