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. Author manuscript; available in PMC: 2020 Oct 8.
Published in final edited form as: Immunohorizons. 2020 Jun 4;4(6):292–307. doi: 10.4049/immunohorizons.2000031

FIGURE 4. Distinct gene expression profile of MR1tet+ MAITs compared with memory CD8+ T cells.

FIGURE 4.

(A) PCA plot illustrating differences between memory CD8+ T cells and MR1tet+ MAITs and between LTBI and TB neg individuals. (B) Volcano plot obtained from the DEseq2 analysis showing log2 fold change versus −log10 p value. The differentially expressed genes are represented in black (adjusted p value <0.01, absolute log2 fold change >1 are indicated by dotted lines). (B) MR1tet+ cells compared with memory CD8+ T cells, (C) CCR1, CXCR4, CXCR6, TIGIT, IL7R, and ABCB1 expression at the mRNA (upper panels: gene expression values in counts normalized by sequencing depth calculated by the DEseq2 package) and protein (lower panels: protein expression as percent frequency of subset) levels in memory CD8+ T cells and MR1tet+ MAITs. Gene expression data were derived from memory CD8+ T cells from 17 individuals and MR1tet+ cells (n individuals = 16) using an Illumina sequencing platform. Protein expression data were derived from memory CD8+ T cells from 20 individuals and MR1tet+ cells (n individuals = 20) using flow cytometry. Median ± interquartile range is shown. Two-tailed Mann–Whitney U test.