FIGURE 1.
Metabolic acidosis (MA) impairs clearance of UPEC‐UTI in mice prone to vesicoureteral reflux (VUR). C3H HeN (5/group) were fed normal rodent chow or rodent chow supplemented with 2% NH4Cl (MA), from day −2 to +3 with respect to bladder instillation of with 5 × 106 cfu/50 µl cfu UPEC strain CFT073 on day 0. For antibody‐mediated depletion of Ly6G/C+ cells, C3H‐HeN mice (5/group) were administered rat IgG2b isotype control 100 µg i.p. (Iso) and/or NIMP‐R14, monoclonal anti‐Ly6G/C 100 µg I.P. aon days −1, 0 and +1.Kidneys were harvested 3 days post‐infection (dpi) and UPEC burden determined by plating serial dilutions of tissue homogenates. Lines delineate average bacterial burden. *p < .02 versus Normal, **p < .05 versus MA isotype control; Mann–Whitney U‐TEST. Similar results were observed in bladder homogenates; data not shown.