FIGURE 3.

Urine acidification does not affect UPEC burden. C3H‐HeN mice (5/group) were fed normal rodent chow (5010, normal), rodent chow 5002 supplemented with 2% NH₄Cl (Acidosis), or 2% NH₄Cl‐supplemented diet in conjunction with 50 mg kg⁻¹ day⁻¹ acetozolamide administered via Alzet® osmotic pump from day −2 to +3 with respect to bladder instillation of with 10⁷ cfu/50 ul UPEC strain CFT073 on Day 0. Kidneys were harvested day +3 PI and UPEC burden determined by plating serial dilutions of tissue homogenates. Lines delineate average bacterial burden. *p < .02 versus Normal; Mann–Whitney U‐TEST. Sham‐N = sham surgery normal diet, MA = Metabolic Acidosis, MA + ACZ = metabolic acidosis in conjunction with 50 mg kg⁻¹ day⁻¹ acetazolamide