TABLE 3.
Kidney neutrophil infiltrate is increased by metabolic acidosis during UPEC‐UTI
| Normal | Metabolic acidosis | Fold | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Live | Dead | %Viable | Total | Live | Dead | %Viable | Total | Diff. | |
| Exp. 1 | 4.8E5 | 4.6E5 | 51.0% | 9.5E5 | 2.3E6 | 1.2E6 | 66.1% | 3.5E6 | 3.7 |
| Exp. 2 | 1.5E5 | 1.1E5 | 57.6% | 2.7E5 | 7.1E5 | 3.9E5 | 64.4% | 1.1E6 | 4.1 |
| Exp. 3 | 6.6E5 | 2.7E5 | 70.6% | 9.3E5 | 4.6E6 | 8.2E5 | 84.8% | 5.4E6 | 5.8 |
Neutrophils were isolated from a pool of supernatants from collagenase digested kidneys (4 kidneys from 2 mice/condition) by magnetic sorting utilizing monoclonal anti‐Ly6G (1A8) as outlined in Figure 4. Neutrophils (live/dead) were enumerated via acridine orange/propidium iodide (AO/PI) staining and analysis utilizing a Cellometer K2 Fluorescent Viability Cell Counter (Nexcelom Bioscience). Fold difference versus normal (Mean ± SE) = 4.5 ± 0.6 p < .01 versus normal, T‐test.