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Effect of NM2A and 2B ablation on tip cell dynamics in EB sprouts. Phase contrast time lapse videos of EB sprouts at 1sec/frame with 40X objective. (a) wild-type sprout, (b) A−/A− sprout, (c) B−/B− sprouts. Wild-type and B−/B− tips cells generate numerous cortical blebs (a,c, arrow). A−/A− tip cells develop cortical ruffles (b, arrow), but no blebbing. 1sec/frame (capture rate), 100fps (playback).
