FIGURE 5:

Constitutively activating PRD1 enhances CME efficiency as well as dynamin recruitment at CCPs. (A) The ability of wild-type and constitutively active (S774/778A) dynamin chimera to rescue TfnR uptake in the absence of endogenous Dyn1 and Dyn2. Data shown are average ± SD (n = 3 independent biological repeats). (B) Quantification of the percentage of Dyn-positive CCPs from dual-color TIRF images of mRuby-CLCa cells expressing eGFP fusions of wild-type or constitutively active dynamin domain swap chimera in the absence of endogenous Dyn1 and Dyn2. (C) Quantification of average Dyn-eGFP intensity in Dyn-positive CCPs from dual-channel TIRF images in the absence of endogenous Dyn1 and Dyn2. The number of CCPs analyzed in B and C is 19,000 CCPs from 40–50 cells/condition in single imaging experiment. The error bars represent SD; t test was used to calculate the statistical significance.