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Knock-in guide sequences.
| Cell line generated | Parental cell line | Guide # | Guide sequence | PAM |
|---|---|---|---|---|
| B16F1_VASP-eYFP | B16F1 | 1 | CCACTTGGAAGATTCCACGT | GGG |
| 2 | CCCACTTGGAAGATTCCACG | TGG | ||
| B16F1_VASP-eYFP_Lpd-TdTomato | B16F1_VASP-eYFP | 1 | GGCTATGCAACATTGCGAAG | AGG |
| 2 | CACTACCTGAATAACATATC | AGG | ||
| B16F1_VASP-eYFP_IRSp53-TdTomato | B16F1_VASP-eYFP | 1 | TTCAGATGGACGTTGGCGAA | GGG |
| 2 | GGCCGATGTGAGGCGAGGCT | AGG |
