Figure 1.

Knockdown of ubc-9 or aos-1 expression in neurons causes increased muscle excitation. Aldicarb assays were performed on animals neuronally sensitized to RNAi. Worms were fed RNAi-containing bacteria targeting gfp as a negative control, (A) ubc-9, or (B) aos-1 from the L1 stage. Approximately 20 young adult RNAi-treated worms per plate were exposed to 1 mM aldicarb and paralysis was measured in response to gentle prodding on the nose with a platinum wire. Representative assays testing 3 plates per strain are shown. Error bars denote s.e.m.