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. 2020 Sep 16:bbaa193. doi: 10.1093/bib/bbaa193

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© The Author(s) 2020. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com

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Detection of SARS-CoV-2 RNA with lower CT value by AP-PCR in specimens from 252 confirmed cases. The CT value obtained with the AP-PCR assay was lower than that obtained with the commercial kit A by 2.27 cycles in analyzing the specimens from Site 1. (A) (n = 33, P = 0.0001, paired t-test) and by 3.79 cycles for the specimens from Site 3. (C) (n = 54, P < 0.0001, paired t-test). B. The CT values obtained with the AP-PCR assay was lower than that obtained with the commercial kit B by 3.74 cycles for the specimens from Site 2 (n = 59, P < 0.0001, paired t-test). D. In specimens from Site 1, AP-based isolation yielded lower Ct value targeting the N (by 1.42 cycles, n = 33, P = 0.043 paired t-test) and ORF (by 1.71 cycle, n = 33, P = 0.007, paired Student’s t-test) gene than that of the commercial RNA isolation. E. In specimen from Site 2, the AP-based RNA isolation also improved SARS-CoV-2 RNA stability (by 2.29 cycles, n = 59, P < 0.0001 paired Student’s t-test).