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. 2020 Oct;48(10):1008–1017. doi: 10.1124/dmd.120.000073

Fig. 4.

Fig. 4.

TDI of CYPs by CBD, THC, 11-OH-THC, and COOH-THC. When the cannabinoids were preincubated with HLMs only, CBD showed TDI of CYP1A2, 2C19, and 3A. Pooled HLMs (0.5 mg/ml protein) were preincubated with NADPH regenerating system, cannabinoid (THC, CBD, 11-OH-THC or COOH-THC; 10 μM), or vehicle (0.2% v/v DMSO) at 37°C for 0, 10, 20, or 30 minutes. Then, an aliquot (10 μl) of this mixture was incubated with NADPH regenerating system and a CYP substrate cocktail consisting of phenacetin (CYP1A2; 50 μM), diclofenac (CYP2C9; 5 μM), omeprazole (CYP2C19; 20 μM), dextromethorphan (CYP2D6; 5 μM), and testosterone (CYP3A; 20 μM) for 15 minutes. Data represent percent of activity in the vehicle-treated control group (0 minutes) that was not subjected to preincubation and are shown as means ± S.D. for three independent experiments. *P < 0.05, significantly different from the vehicle-treated control group (two-way analysis of variance test).