Figure 3.

Decrease of Lymphocyte Frequencies with Differentiated and Activated Cytotoxic Phenotype in ARDS Patients at the Initial Visit

Peripheral blood from 37 patients from the COVID-19 control group (n = 27, in white) or ARDS group (n = 10, in gray) was subjected to evaluation for differentiation (A-D) and activation state of T cell (E-L) and B cell (M-O) subsets using multiparametric flow cytometry. The subsets of the CD3⁺ T cells and the CD19⁺ B lymphocyte were identified according to the gating strategy depicted in Figures S8–S10. In all cases, the left boxplots show the data for the initial visit, while the right boxplots depict the data at follow-up. The shaded area indicates the reference range as detected in healthy individuals. Boxplots depict the median and the first and third quartiles. The whiskers correspond to 1.5 times the interquartile range.