Figure 1.

(A) Detection and monitoring of anti-NF-155 and NF-186 antibodies in flow cytometry using transfected cells according to the method described previously (1). Mean fluorescence intensity is reported in the figure (number per row) and the presence of antibodies is also visible by the decrease in the area under the curve. In black, you can see a control subject (without IgG4 nor IgM antibodies). Each color corresponds to a date (reported below). On the left, you can see the IgG4 serotype with no specific antibodies. On the right, the IgM serotype was reported, for NF155 (up) and NF186 (down). The intensity of fluorescence intensity for anti-NF155 and NF186 IgM antibodies decreased after plasmapharesis (in purple), but not after immunoglobulins therapy (in orange), compared to the initial examination on the day of Guillain-Barré syndrome onset (in red). (B) Longitudinal ultrastructure of left peroneal nerve: node of Ranvier slightly wide. In the paranodal region: loss of cohesion of the outermost myelin sheath. Terminal loops are partially disrupted and distant from the axolemma (arrows), whereas myelin is compacta and not disorganized (C).