Fig 2. Analysis of gene expression in PAs isolated from the cerebral cortices of adult Mlc1-EGFP mice.

(A); Schematic summarizing the experimental strategies for isolating cells from cerebral cortices of adult (P30) Mlc1-EGFP knock-in mice. Brain tissue was dissociated and live PAs were fractionated based on EGFP expression using FACS-based approaches. Single cells were subsequently analyzed using quantitative RNA sequencing. (B); Unsupervised clustering of single cell RNA sequencing data using EGFP-expressing PA cells fractionated from mouse cerebral cortices. Cells are clustered based on shared similarities in mRNA expression, with each of the two main clusters representing a subpopulation of PAs showing differential, but somewhat overlapping gene expression. (C); Feature plots showing the expression of select genes with established roles in mouse brain astrocytes including Mlc1 Aldh1l1, and Slc1a3. Each dot indicates a distinct mRNA sequencing result from a single cell. Red indicates higher gene expression and grey indicates lower gene expression.