Fig 4. Quantitation of PAs and non-PAs within the cerebral cortices of Mlc1-EGFP;GLAST-DsRed adult mice.

(A-C); Coronal brain sections through the cerebral cortices of adult Mlc1-EGFP;GLAST-DsRed mice were immunolabeled with anti-GFP antibodies (A) to visualize Mlc1-expressing cells (PAs) and anti-RFP antibodies (B) to visualize cells expressing DsRed via the Slc1a3 promoter (PAs and non-PAs). In the merged confocal image (C), note that some cells express both EGFP⁺ and DsRed⁺, whereas other cells are single positive for each fluorescent marker. Scale bar, 50 μm (A) and 10 μm (inset, C). (D); Quantification of the EGFP⁺ and RFP⁺ single positive cells as well as EGFP⁺/RFP⁺ double positive cells in the cortices of Mlc1-EGFP;GLAST-DsRed mice. Numbers of fluorescence positive cells are plotted as a percentage of total numbers of cells based on DAPI labeling, although DAPI images have not been included in this figure. Note that more than 20% of cells within the adult cerebral cortex are EGFP⁺ or EGFP⁺/RFP⁺, whereas non-PAs constitute approximately 20% or more of total cells. The red bars indicate DsRed⁺/EGFP^- cell fractions.