Table 6. Restriction endonuclease analysis and polymorphisms in fragment length.
| Genome | BamHI | HindIII | KpnI | PvuII | XhoI | cp type |
|---|---|---|---|---|---|---|
| GON1, GON2, PHU, STN, BUK, CUR | 15.6 kb + 3.76 kb → 19.4 kb | 2.58 kb—48 bp → 2.54 kb | - | - | - | S |
| ADG2, CHA | 15.6 kb + 3.76 kb → 19.4 kb & 3.88 kb—297 bp → 3.59 kb | - | - | - | - | A |
| ADG1, AJH, JUZ | 15.6 kb + 3.76 kb → 19.4 kb | - | - | - | - | C |
| TBR | - | - | - | - | - | W |
| BUK2 | - | - | - | 2 X 20.9 kb → 41.8 kb | - | W2 |
Different polymorphisms were observed in fragment lengths when digested with five restriction enzymes. These length polymorphisms were compared with previous studies to identify plastome types [1, 10]. The RFLP analysis confirms the findings from the marker analysis and also identify the types of ADG2, CHA, BUK2, and TBR.