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. 2020 Sep 28;16(9):e1009004. doi: 10.1371/journal.pgen.1009004

Fig 5. Knockout of BmCoc in silkworm by CRISPR/Cas9 system.

Fig 5

(A) Gene structure with gRNA targeting exon 3 of BmCoc. (B) qRT-PCR analysis of the relative expression level of BmCoc in the wildtype (BmCoc+/+) and homozygous individuals (BmCoc-/-) on day 8 of the pupal stage, using cDNAs of heads as templates. The expression level was compared between BmCoc-/- and BmCoc+/+ by two-tailed Student’s t-tests. ** P < 0.01. (C) The SDS-PAGE analysis for the proteins contained in the silkmoth-vomiting fluid collected from wild type and BmCoc-/- individuals. The gel was stained with Coomassie brilliant blue R-250. The protein band indicated by the red arrow is the mature cocoonase with a predicted molecular weight of 23.7 kDa. M indicates a protein marker and H1-H8 lanes represent different fluid samples from eight homozygous individuals.