Figure 2.

Single-stranded target DNA binding and periodic cleavage by the I-D effector. (A) Electrophoretic mobility shift assay showing complex formation between radiolabelled ssDNA and the type I-D effector; ssTS_CAC: target ssDNA with CAC at PAM position; ssTS_TTT: target ssDNA with TTT at PAM position. (B–D) ssDNA cleavage by type I-D effector. The radiolabelled ssDNA targets are shown on top of each gel under which the presence (+) or absence (+) of the type I-D components is indicated. The sizes of cleavage products are depicted on the right side of each gel. Unsp.: Unspecific cleavage. Cas10d^HD_: HD mutant of Cas10d. (E) Schematic depiction of the cleavage sites on ssDNA targets.