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Fluorescent gel images of standing-start primer extension reactions with HIV-1 RT using native (A), m4C-modified (B), and m42C-modified (C) RNA strands as templates. Lanes: L, reference DNA 20mer ladder; P, primer; Nat, natural template with all four dNTPs as positive controls in each gel; A, T, G and C, reactions in the presence of the respective dNTP only; N, reactions in the presence of all four dNTPs.
