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. 2020 Sep 17;48(18):10576–10589. doi: 10.1093/nar/gkaa764

Figure 5.

Figure 5.

Effect of the lengths of the RT template and PBS in the pegRNA on the efficiency of on-target and off-target editing in HEK293T cells. (A and B) The dependence of the PE2 specificity on the lengths of the PBS or RT template was investigated using HBB-targeted pegRNAs with variable PBS lengths (10–16 bp) or RT lengths (11–17 bp), respectively (A and B). (C andD) Representative graphs showing relative frequencies (off-target activity/on-target activity) depending on the PBS length (C) or the RT template sequence length (D). Error bars indicate the s.e.m. (three biologically independent samples).