Figure 7.

Specificity of PE3. (A and B) Plasmids encoding PE2, pegRNA and sgRNA were transfected into HEK293T cells and the frequencies of precise edits and unwanted indels were measured using targeted deep sequencing at the HEK4 (A) and HBB (B) on-target and validated off-target sites. The mismatched nucleotides are shown in red lowercase letters and the PAM sequences are shown in bold font. PBS and RT template sequences are highlighted with blue and orange backgrounds, respectively. Error bars indicate the s.e.m. (three biologically independent samples).