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. 2020 Sep 22;48(18):10456–10469. doi: 10.1093/nar/gkaa743

Figure 1.

Figure 1.

Summary of NCBP interactions and methodological approach. (A) NCBP interactions. Right-side pathway: The canonical CBC consisting of NCBP1 and NCBP2 engage in early interactions guided by transcription, splicing, and 3′-end processing, to RNA export or decay (example interactors are shown for each summarized process; see main text for details). Left-side pathway: Putative NCBP3 functional interactions include: participation in splicing (16–18,20); formation of an alternative CBC with NCBP1; and contributions to mRNA export (16). NCBP3 interacts with CBC and ARS2 (5,16) and is affiliated with the EJC, and TREX complexes (16–18,20). (B) Methodological approach. Cryomilled cell powders are distributed with a dispensing manifold and macromolecules are extracted with 24 different extraction solutions (1). Brief sonication is applied to disperse and homogenize the extracts (2). After clarifying the extracts by centrifugation, affinity capture is performed (3) and protein eluates are subjected to MS analysis (4) and data processing (5).