Figure 5.

Global analysis contextualizes NCBP interactor relationships - multidimensional scaling (MDS). Select protein complexes are shown in a static 3D MDS plot (interactive plot: https://ncbps.shinyapps.io/3d_mds_app/). This is a composite analysis of all IPs and all interacting proteins. Three panels selectively display some groups while omitting others for clarity in reading the figure labels but all nodes are simultaneously present in the analysis at the positions indicated. The colour scheme for different groups of proteins is illustrated in the center of the plot. Panel (A) displays the segregation of the NCBPs, SRRT, PHAX, the EJC, and THO/TREX proteins. Panel (B) labels, CBCAP, spliceosomal, and NPC proteins with text; the nodes displayed for NCBP3, EJC, and THO/TREX in panel (A) are also included in (B) for positional context, without labels to avoid crowding. These complexes can also be viewed in 3D context with rotation in the supplemental file: 3D_Animated_MDS.gif. Panel (C) displays the segregation of the NCBPs, SRRT, PHAX, the EJC, THO/TREX, NPC, and importins.