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. 2020 Sep 17;48(18):10241–10258. doi: 10.1093/nar/gkaa745

Figure 6.

Figure 6.

Spn1 is required for normal localization of histone H3 modifications along coding genes. (A) Top: Average H3-normalized H3K36me2 for 3087 non-overlapping, verified coding genes aligned by TSS in non-depleted and Spn1-depleted conditions. The solid line and shading are the median and interquartile range of the mean ratio over two replicates. Vertical dotted lines indicate the distances at which the median signal in each condition reached 90% of its maximum value. Bottom: Heatmap of the change in H3-normalized H3K36me2 enrichment upon Spn1 depletion for the same genes, aligned by TSS and arranged by transcript length. Data is shown for each gene up to 300 bp 3′ of the cleavage and polyadenylation site, which is indicated by the white dotted line. (B) Same as in (A) but for H3K36me3. (C) Same as in (A) but for H3K4me3, with vertical dotted lines instead indicating the distances at which the median signal in each condition dropped to 10% of its maximum value.