Figure 1.

Time-resolved RNA profiling within 24 h of CD4+ T cell activation. (A) Schematic overview on the experimental setup. Peripheral CD4+ T cells were isolated from the blood of two healthy, age and gender matched donors. The cells were in vitro activated by antibody-coupled beads. RNA samples were collected from three independently activated samples per time point and donor at intervals of 2 h and over a total time period of 24 h (n = 78 samples). Expression courses of miRNAs and mRNAs were determined by microarray-based profiling. (B) The total RNA yield of each time-course sample is shown for each time point. (C) T cell activation pathways (GO terms) were significantly upregulated, comparing the time-resolved mRNA expression data after T-cell activation to the expression values at 0 h (P-values adjusted by Benjamini−Hochberg). At each time point, three samples were analyzed for each donor.