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. 2020 Sep 29;48(18):10164–10183. doi: 10.1093/nar/gkaa788

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Quantification of absolute miRNA molecules per cell in context with CD4+ T cell activation. Absolute miRNA expression [molecules/cell] was determined for all detected miRNAs by the application of a microarray calibration curve to the time-resolved expression data. The quantitative and time-resolved miRNA patterns were evaluated based on the median result of all RNA samples from donors 1 and 2 (n = 6 per time point). (A) Total miRNA expression was determined by the sum of all detected miRNAs at 0 and 24 h after activation (n = 815). (B) The maximum expression of each miRNA was determined for the total 24 h time frame. (C) The highest expressed miRNAs were compared between the 0, 12 and 24 h time points. The increase of miR-451a was during 7–8 h after activation and is therefore not represented by the displayed time points.