Table 1.
NGS analysis of RNase A and RNase A / RNase III digested HEK293 RNA. HEK293 RNA was subjected to RNase A and RNase A / RNase III digestion and size-fractionated (samples A and A/III from Figure 1C, upper panel). RNA isolations were used for cDNA library preparation and sequence analysis. Identified sequences were mapped to the 45S rRNA (NR_046235.1) and number of identified reads in each sample as well as their ratio are depicted
| Number of reads | ||||||
|---|---|---|---|---|---|---|
| Start | End | length | Sequence | RNase A | RNase A/III | RNase A / RNase A/III |
| 28S rRNA | ||||||
| 8698 | 8751 | 54 | GUCCGUCCGUCCUCCUCCUCCCCCGUCUCCGCCCCCCGGCCCCGCGUCCUCCCU | 12 | 0 | - |
| 8694 | 8751 | 58 | GUCCGUCCGUCCGUCCUCCUCCUCCCCCGUCUCCGCCCCCCGGCCCCGCGUCCUCCCU | 47 | 0 | - |
| 8690 | 8751 | 62 | GUCCGUCCGUCCGUCCGUCCUCCUCCUCCCCCGUCUCCGCCCCCCGGCCCGCCGUCCUCCCU | 101 | 0 | - |
| internal transcribed spacer 2 (ITS2) | ||||||
| 7222 | 7290 | 69 | GGUGGGGGAGGAGGAAGGCGGGUCCGGAAGGGGAAGGGUGCCGGCGGGGAGAGAGGGUCGGGGGAGCGC | 104 | 1 | 104.0 |
| 7221 | 7290 | 70 | UGGUGGGGGAGGAGGAAGGCGGGUCCGGAAGGGGAAGGGUGCCGGCGGGGAGAGAGGGUCGGGGGAGCGC | 16 | 0 | - |
| 7220 | 7291 | 72 | GUGGUGGGGGAGGAGGAAGGCGGGUCCGGAAGGGGAAGGGUGCCGGCGGGGAGAGAGGGUCGGGGGAGCGCA | 32 | 0 | - |
| 7219 | 7290 | 72 | GGUGGUGGGGGAGGAGGAAGGCGGGUCCGGAAGGGGAAGGGUGCCGGCGGGGAGAGAGGGUCGGGGGAGCGC | 652 | 7 | 93.1 |
| 7219 | 7288 | 70 | GGUGGUGGGGGAGGAGGAAGGCGGGUCCGGAAGGGGAAGGGUGCCGGCGGGGAGAGAGGGUCGGGGGAGC | 10 | 1 | 10.0 |
| 7218 | 7291 | 74 | UGGUGGUGGGGGAGGAGGAAGGCGGGUCCGGAAGGGGAAGGGUGCCGGCGGGGAGAGAGGGUCGGGGGAGCGCA | 21 | 0 | - |
| 7217 | 7290 | 74 | GUGGUGGUGGGGGAGGAGGAAGGCGGGUCCGGAAGGGGAAGGGUGCCGGCGGGGAGAGAGGGUCGGGGGAGCGC | 534 | 2 | 267.0 |
| 18S rRNA | ||||||
| 5380 | 5426 | 47 | GGUUUAGUGAGGCCCUCGGAUCGGCCCCGCCGGGGUCGGCGGAGGGC | 46 | 46 | 1.0 |
| 5376 | 5427 | 51 | GGAUGGUUUAGUGAGGCCCUCGGAUCGGCCCCGCCGGGGUCGGCGGAGGGC | 398 | 284 | 1.4 |
| 5376 | 5419 | 44 | GGAUGGUUUAGUGAGGCCCUCGGAUCGGCCCCGCCGGGGUCGGC | 2340 | 2213 | 1.1 |