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. 2020 Sep 29;48(18):10101–10124. doi: 10.1093/nar/gkaa750

Table 2.

Percent C3′-endo conformers of 5′-O-TBDPS-protected 2′,3′-diols of 5′-(R)- and 5′-(S)-C-methyl-guanosine based on 1H NMR

Compound 5′-O protection 5′-C modification 3 J H1′-H2′ (Hz) % C3′ endoa
Guanosine TBS H 5.3 ∼50
(R) isomer, 5 TBDPS methyl 6.6 ∼30
(S) isomer, 15 TBDPS methyl 5.3 ∼50

aThe percent of molecules in the C3′-endo conformation was calculated as (100 – (10 × 3JH1′-H2′)) as previously described (52), where 3JH1′–H2′ is the 1H NMR coupling constant of the sugar H1′ and H2′ protons.